Tiangen plasmid extraction kit
WebbThe protocol is about using TIAN prep Mini Plasmid Kit II (TIANGEN Code No. DP106-02) to extract plasmid. Before starting 1. Solution P1 was added to RNase A before use (all … Webb8 apr. 2024 · The collected samples were immediately snap frozen in liquid nitrogen and stored at -80°C. The RNA of the samples was extracted with reference to the instructions …
Tiangen plasmid extraction kit
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Webbför 2 dagar sedan · Instead of plasmid or extracted DNA, tissue of shrimp as testing sample has advantages in contributing to inspect all steps of practical testing detection, … WebbIn the instructions for the Tiangen kit, it recommends a maximum of 5 minutes in lysis solution (step 4). I recommend doing this step quickly, e.g. within 10 seconds, or as soon …
Webb13 apr. 2024 · DNA gel extraction kits, plasmid extraction kits, and nucleic acid purification kits were purchased from TIANGEN (China). Ni 2+ -NTA agarose affinity column, beechwood xylan (X4252-25G) and xylose were obtained from Sigma-Aldrich (USA). Webbsheep blood using a DNA extraction kit (Tiangen, Beijing, China) according to the manu-facturer’s instructions. Its purity was detected using 1.5% agarose gel electrophoresis, and its content was detected using an ultraviolet spectrophotometer. Extracted DNA was stored at -20 ºC. 2.3. Lin28B promoter sequence analysis and vector construction
Webb6 apr. 2024 · The DNA/RNA extraction kit and reverse transcription kit were purchased from TaKaRa ... (TaKaRa, Dalian, China) for DH5α (TIANGEN) transformation. After … Webbför 2 dagar sedan · Phage-plasmids are extra-chromosomal elements that act both as plasmids and as phages, whose eco-evolutionary dynamics remain poorly constrained. Here, we show that segregational drift and loss-of ...
WebbThe PCR products were then separated by 1.5% agarose gel electrophoresis, and purified by a Gel Extraction Kit (Tiangen, Beijing, China). The purified PCR products were cloned …
WebbTIANpure Midi Plasmid Kit_Plasmid DNA & DNA Clean Up_Product_TIANGEN Room temperature (15-25℃) Description The TIANpure Midi Plasmid Kit adopts a specific … game time for astrosblack hawthorn bushWebbcollected from 3-5 plants for DNA extraction which was performed with Tiangen DNA Secure Plant Kit. PCR system (20 μL total volume) included 1 μL DNA, 1 μL of both forward and reverse primers, 10 μL Taq 2× master mix and 7 μL sterilized deionized water. PCR procedures were as follows: 10 min predenaturation at 95°C; 30 s game time for hockey tonightWebbExtraction of Bacterial Genomic DNA. The plasmid DNA of N. gonorrhoeae was extracted using a spin column-type plasmid mini-extraction kit DP103-03 (Tiangen Biochemical … game time food truckWebb12 apr. 2024 · The fecal DNA was isolated using TIANGEN stool DNA kit (Beijing, China) according to the manufacturer’s recommendations. For 16S amplicon sequencing, the variable V4 region of bacterial 16S rRNA was amplified with primers V4F (5’-GTGTGYCAGCMGCCGCGGTAA-3’) and V4R (5’-CCGGACTACNVGGGTWTCTAAT-3’), and … game time for big 10 championshipWebbFor fast purification total RNA from cells and tissues using gDNA Eliminator columns or plates. QIAamp Circulating Nucleic Acid Kit. For isolation of free-circulating DNA and RNA from human plasma or serum. miRNeasy Tissue/Cells Advanced Kits. For phenol-free purification of micro RNA and total RNA from tissues and cells, including small samples. black hawthorn flowersWebbThe HighPure Maxi Plasmid Kit adopts a unique buffer system incorporation with TIANGEN's special color indicator to ensure the extraction of large amount of high-purity … game time for houston astros today