WebJan 28, 2014 · In an attempt to develop a variety of expression vector systems for Corynebacterium glutamicum, six types of promoters, including P tac, P sod, P sodwith a … Webseveral expression vector systems based on pCES208 plasmids were constructed. GFP was expressed in C. glutamicum under the control of increasing repeats of P vgb (P vgb, P vgb4, P vgb8). The strength of protein expression in the three constructs was investigated by measuring the intensity of fluorescence
Development of a potential stationary-phase specific gene
WebUsing the vector, the expression of pyrR from C. glutamicum was tried by temperature shift-up. The results indicated that the constructed vectors (pCeHEMG) can be successfully used in the expression and regulation of foreign genes in corynebacteria. From the journal Journal of Microbiology and Biotechnology ISSN : WebNov 19, 2024 · Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a... florist in hamilton alabama
US11111485B2 - Expression system for Psicose epimerase and …
WebApr 19, 2024 · coli strain BL21, and the three GRAS hosts strains were constructed with different vector systems —pGEX 4T-1, pCES208, pYES 2.1 and pNZ8148, respectively. … WebThe present invention relates to a gene expression cassette capable of producing psicose at high yield with high stability, a GRAS (Generally recognized as safe) microorganism, a method of... With C. glutamicum producing eGFP, we performed an adaptive laboratory evolution based on the fluorescence intensity of each cell. C. glutamicum harboring pCES-H36-GFP in which the eGFP gene was expressed under the strong constitutive promoter (PH36) were cultivated and the cells exhibiting higher … See more After the seventh round screening, the sorted cells were spread on an agar plate and 10 individual clones were randomly picked for an analysis of eGFP … See more To verify that the nonsense mutation in the parB gene contributed to the increase in the plasmid copy number, we introduced a point mutation (C→A at the 21st … See more In general, the maintenance of high-copy-number plasmids in bacteria can give high metabolic load on the hosts, which consequently causes poor cell … See more To demonstrate the versatility of the high-copy-number plasmids, we examined the secretory production of endoxylanase from Streptomyces coelicolor which can … See more florist in harrison oh