WebApr 7, 2003 · EA.hy926 cells were E2-deprived for 48 h in phenol red- and fatty acid-free DMEM in the presence of 10% gelding horse serum, and quiesced for 12 h in the … WebEA.hy926 cell line was purchased from ATCC (ATCC CRL-2922). EA.hy926 cells were grown in DMEM medium supplemented with 10% fresh bovine serum in a humidified atmosphere containing 5% CO 2 at 37 °C. Cultured cells without treatment served as controls. Confocal fluorescence imaging methods
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WebJan 25, 2024 · EA.hy926 cells were cultured in 75 cm 2 flasks in Dulbecco’s modified Eagle’s medium (DMEM) containing 1 g/L glucose, 3.97 mM L-glutamine, and 1 mM sodium pyruvate supplemented with 10% (v/v) fetal calf serum (FCS), 100 U/mL penicillin, 100 μg/mL streptomycin, and 1x HAT supplement (v/v) at 37°C (under a 5% CO 2 … WebEA.hy926 cells (ATCC, USA), monoclonal rabbit phospho AKT-Ser 473, phospho eNOS Ser-1177 antibodies, polyclonal secondary goat anti-rabbit, and anti-mouse antibodies were from cell signalling technology. Dubelcco’s Modified Eagles Medium (DMEM), fetal bovine serum, penicillin, streptomycin, angiotensin II, polyclonal
WebOct 25, 2024 · EA.hy926 cells culture and proliferation assay . Human umbilical vein EA.hy926 cells (Cell bank, Shanghai Institute for Biological Science, Shanghai, China) were cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, CA, USA) at 37°C in a … WebJul 22, 2024 · EA.hy926, LPS-induced sepsis HUVECs, LPS1, 12h: Sample type: SRA : Source name: EA.hy926: Organism: Homo sapiens: Characteristics: cell line: EA.hy926 …
WebAug 29, 2024 · 29 Aug 2024 by Datacenters.com Colocation. Ashburn, a city in Virginia’s Loudoun County about 34 miles from Washington D.C., is widely known as the Data …
WebApr 21, 2024 · Visitor Information on COVID-19 Precautions. We strongly recommend making an appointment with the FSDO before coming to the office.. In accordance with … high bridge ny zip codeWeb(A) EA.hy926 cells were cultured in serum-free DMEM, serum-free DMEM with 100 ng/ml CHI3L1, serum-free DMEM with 100 ng/ml IL-8 and serum-free DMEM with 50 ng/ml … highbridge nyc condosWebMay 28, 2024 · EA.hy926, LN229 and A172 were cultured in DMEM or DMEM GlutaMax (Gibco, Life Technologies Corporation, Paisley, UK) supplemented with 10% fetal calf serum (FCS; Gibco, Life Technologies Corporation, Paisley, UK), and for EA.hy926 with 1% HAT. All cells were maintained at 37 °C in a humidified atmosphere containing 5% CO 2. To … how far is ohio to michiganWebApr 1, 2024 · Human EA.hy926 vascular endothelial cells were cultured in DMEM/F-12 medium and human peripheral blood monocyte (THP-1) cells were cultured in RPMI … how far is ohio to marylandWebMar 11, 2014 · EA.hy926 and HepG2 cells were cultured in DMEM supplemented with 10% (v/v) heat-inactivated fetal calf serum, penicillin (100 U/ml) and streptomycin (100 U/ml) (both Sigma-Aldrich). Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO 2 and 95% air. The medium was changed every two days. Cell … high bridge nj townshipWebOct 31, 2024 · EA.hy926 endothelial cells were seeded into a 96-well plate at the density of 3 × 104 cells per well with various concentrations of leptin (0, 0.1, 1, 5, 10, or 50 ng/ml). Premixed CCK-8 (10 μl) and medium were … highbridge nursing homeWebThe EA.hy926 cells did not proliferate on the bare NiTi controls. Poor proliferation was also observed on abraded samples Nos. 1, 3, and 5. ... (DMEM/F12 supplemented with 10% FCS, 2 mM L-glutamine, and 40 μg/mL gentamicin) for 72 h at 37 °C, and had a surface area/volume ratio of 1.25 cm −1. Subsequently, the samples were removed from the ... how far is ohio to florida