Adipocyte rna-seq

Author: fmkq

August undefined, 2024

WebSep 16, 2024 · The transcriptome data was generated from the different stages of the 3T3-L1 adipocytes using RNA-seq technology. Table 2 briefly summarizes the information of … WebApr 12, 2024 · IMF deposition primarily occurs through the increase in the numbers of preadipocytes (cell proliferation) and adipocyte size (cell enlargement) . Intramuscular preadipocytes ... and stored in a −80 °C refrigerator for later RNA-seq, and part of the samples were placed in an incubator in an ice pack and then quickly sent to the …

Isolation of nuclei from mouse white adipose tissues for single …

WebJan 22, 2024 · The RNA was extracted on the 25th day (10 days of osmotic compression and 14 days of colony expansion) for RNA-seq. Library construction of digital gene expression sequencing generated tags with 21 base pairs (bp) from the 3′ ends of each transcript, and these tags were used to represent the expression levels of transcripts. WebApr 19, 2024 · RNA sequencing (RNA-Seq) was conducted to explore the chicken transcriptome from the thigh muscle tissue of 6 Huangshan Black Chickens with 3 extremely high and low phenotypic values for percentage of polyunsaturated fatty acids (PUFAs). heading gps

The Impact of Single-Cell Genomics on Adipose Tissue Research

WebAug 8, 2024 · RNA extraction and RNA-seq analysis were performed by NovoGene (Beijing, China), the sequencing platform was illumina novaseq 6000, and the read length was 150bp. The sampling time points were listed in Figure 1. FeatureCounts (1.5.0-p3) is used to calculate the reads mapped to each gene. WebFeb 8, 2024 · RNA-seq was used to determine genes regulated by EGCG and identify the key differences between the two functionally distinct adipose tissues. Taken … WebMar 8, 2024 · We performed RNA-Seq on adipocytes isolated from three different adipose depots (BAT, iWAT, and eWAT) in HFD and ND. More than 200 million paired-end reads … heading grass

Single-cell transcriptional networks in differentiating …

Single-cell analysis of human adipose tissue identifies depot- and ...

WebMar 25, 2024 · However, single-nuclei RNA sequencing (sn-RNAseq) and spatial transcriptomics have created an avenue to explore the complexity of adipocyte status and function in vivo. While current knowledge keeps expanding, it is now clear that distinct adipocyte populations exist within WAT depots. WebApr 8, 2024 · Cesarean section (CS) confers increased risk of type I diabetes, asthma, inflammatory bowel disease, celiac disease, overweight and obesity, etc., in the offspring. However, the underlying mechanism remains unknown. To investigate the influence of CS on gene expression in cord blood, we have performed RNA-sequencing followed by … goldman sachs internship hoursWebApr 11, 2024 · To systematically dissect the transcriptomic differences between homeostasis and chronic dry skin at the single-cell level, we carried out scRNA-seq on two biological mixed samples from each group, and each mixed sample contained three mice (Fig. 1 A).After quality control, we obtained 18,578 cells in the AEW groups and 24,160 cells in … heading graphic design

"WebHere we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. We identify a rare subpopulation of adipocytes in … " - Adipocyte rna-seq

Adipocyte rna-seq

A single-cell atlas of human and mouse white adipose

WebNov 7, 2024 · RNA was prepared from eight different cell WAT cell types (ASCs, total adipose tissue macrophages (ATMs), M1 ATMs, M2 ATMs, total T cells, CD4 + T cells, CD8 + T cells, and mature adipocytes). Ten nanograms of RNA was amplified using four cycles and loaded onto Clariom™D microarray chips. For details see Additional file 1: … WebMar 27, 2024 · The KEGG metabolic pathway suggested a potential role for arachidonic acid metabolism, serotonergic synapses, neuroactive ligand-receptor interactions, tyrosine metabolism, and regiomelanin. Vitamin digestion and absorption, as well as regulation of lipolysis in adipocytes, were the main metabolic pathways that distinguished the T vs. M …

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WebApr 11, 2024 · Background: Insulin resistance (IR) is a major contributing factor to the pathogenesis of metabolic syndrome and type 2 diabetes mellitus (T2D). Adipocyte metabolism is known to play a crucial role in IR. Therefore, the aims of this study were to identify metabolism-related proteins that could be used as potential biomarkers of IR and … WebApr 30, 2024 · a Single-cell RNA seq was performed on differentiating preadipocytes beginning at 80% confluency and differentiated for 7 days. PAGODA was used to …

WebMar 11, 2024 · For RNA-seq, RNA was extracted from about 30 mg of each individual adipose tissue. Equal amounts of RNA were pooled to obtain 6 independent replicates/conditions that were processed for RNA-seq. As depicted in Fig. S1D, each chromatin pool was paired with 3 RNA-seq pools (adipose tissue coming from the same … WebG protein-coupled receptors (GPCRs) are expressed essentially on all cells, facilitating cellular responses to external stimuli, and are involved in nearly every biological process. Several members of this family play significant roles in the regulation of adipogenesis and adipose metabolism. However, the expression and functional significance of a vast …

WebNov 8, 2024 · The snRNA-seq data of digested adipocytes from inguinal WAT reveals a complex subpopulation of mature adipocytes with distinct genetic signatures [ 35 ]. Recently, snRNA-seq analysis of the WAT identified a rare subpopulation of adipocytes in mice that increase in abundance at higher temperatures. WebOct 31, 2024 · On the basis of the aforementioned scRNA-seq results, two up-regulated genes in the intramuscular adipocyte cluster— APOA1 and COL1A1— were selected and verified by RNA in situ hybridization in breast muscles. The known adipocyte marker gene ADIPOQ [ 9] was tested as positive control.

WebWe analyzed adipose gene expression using RNA sequencing in the fenofibrate and omega-3 fatty acid modulation of endotoxemia (FFAME) trial of healthy individuals at three …

WebSeveral studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we … heading h6WebApr 30, 2024 · Single-cell RNA-seq profiles of human preadipocytes during adipogenesis in vitro identifies at least two distinct classes of subcutaneous white adipocytes. These … heading hall ouWebNov 8, 2024 · Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their … heading hallWebJul 11, 2024 · RNA sequencing followed by whole genome–wide expression analysis shows that beige adipocytes induced from bat aWAT, rather than sWAT, have molecular signatures resembling those of mouse sWAT-induced beige adipocytes and exhibit dynamic profiles similar to those of classical brown adipocytes. goldman sachs internship offer emailWebOct 28, 2024 · To comprehensively characterize mature subpopulations of brown adipocytes, we performed single-nucleus RNA sequencing (snRNA-seq) 12, 13 in … goldman sachs internship job descriptionWebDec 3, 2024 · Transcriptome-wide m 6 A-Seq Reveals Global m 6 A Modification Patterns During Yak Adipocyte Differentiation. The yak adipocyte and preadipocyte of three biological replicates were used for transcriptome-wide m 6 A-sequencing (m 6 A-seq) and RNA-sequencing (RNA-seq) assays. In total, 12 libraries were sequenced, comprising … goldman sachs internship hong kongWebJul 18, 2024 · Single-cell RNA-sequencing (scRNA-seq) in mice identified three ASC subpopulations that occupied distinct tissue locations. Of these, interstitial ASC1 were found to be direct precursors of new brown adipocytes (BAs). heading guidance